Frequency of BCR-ABL1, E2A-PBX1, MLL-AF4, and TEL-AML1 Fusion Genes in B-Cell Acute Lymphoblastic Leukemia

Authors

    Francisco Sánchez-Pinto, Gioconda Manassero, Mónica Carola Correa Guerrero, Guillermo Frank Romero Guerra, Julissa Fuentes Vera Instituto Nacional de Salud del Niño de San Borja (INSNSB), Lima 15037, Peru Universidad Peruana Cayetano Heredia, Lima 15102, Peru Instituto Nacional de Salud del Niño de San Borja (INSNSB), Lima 15037, Peru Instituto Nacional de Salud del Niño de San Borja (INSNSB), Lima 15037, Peru Instituto Nacional de Salud del Niño de San Borja (INSNSB), Lima 15037, Peru

Keywords:

Lymphoblastic leukemia, Pediatrics, Molecular biology, Cytogenetics, Immunophenotyping

Abstract

Objective: To describe the frequency of BCR-ABL1, E2A-PBX1, MLL-AF4, and TEL-AML1 fusion genes in patients with B-cell acute lymphoblastic leukemia at the Instituto Nacional de Salud del Niño of San Borja, Lima, Peru. Methods: We studied 375 samples from pediatric patients diagnosed with B-cell acute lymphoblastic leukemia, which were received at the Genetics Service. BCR-ABL1, E2A-PBX1, MLL-AF4, and TEL-AML1 fusion genes were detected in bone marrow or peripheral blood using reverse transcriptase polymerase chain reaction (RT-PCR). Results: Of the total samples, 58 were positive for BCR-ABL1, E2A-PBX1, MLL-AF4, and TEL-AML1 fusion genes, with the following frequencies: 2.13%, 4.53%, 0.53%, and 8.27%, respectively. Conclusions: The TEL-AML1 fusion gene is the most frequent in our pediatric population and is associated with a favorable prognosis.

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Published

2024-04-01