Frequency of BCR-ABL1, E2A-PBX1, MLL-AF4, and TEL-AML1 Fusion Genes in B-Cell Acute Lymphoblastic Leukemia
Keywords:
Lymphoblastic leukemia, Pediatrics, Molecular biology, Cytogenetics, ImmunophenotypingAbstract
Objective: To describe the frequency of BCR-ABL1, E2A-PBX1, MLL-AF4, and TEL-AML1 fusion genes in patients with B-cell acute lymphoblastic leukemia at the Instituto Nacional de Salud del Niño of San Borja, Lima, Peru. Methods: We studied 375 samples from pediatric patients diagnosed with B-cell acute lymphoblastic leukemia, which were received at the Genetics Service. BCR-ABL1, E2A-PBX1, MLL-AF4, and TEL-AML1 fusion genes were detected in bone marrow or peripheral blood using reverse transcriptase polymerase chain reaction (RT-PCR). Results: Of the total samples, 58 were positive for BCR-ABL1, E2A-PBX1, MLL-AF4, and TEL-AML1 fusion genes, with the following frequencies: 2.13%, 4.53%, 0.53%, and 8.27%, respectively. Conclusions: The TEL-AML1 fusion gene is the most frequent in our pediatric population and is associated with a favorable prognosis.
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